6-Carboxyfluorescein ≥98% (by HPLC) fluorescent pH indicator

Supplier: Biotium
Warning

Synonyms: 3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid, 6-FAM

51020
51020.EA 186 EUR
51020.
6-Carboxyfluorescein ≥98% (by HPLC) fluorescent pH indicator
6-Carboxyfluorescein

6-Carboxyfluorescein has a pKa of 6.5 and can be used as a pH sensor. The excitation spectrum of the dye responds to pH similarly to that of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.


  • λEx/λEm (pH 9) = 495/519 nm
  • pKa 6.5
  • Yellow orange solid soluble in water (pH>6), DMF or DMSO
  • Store at 4°C and protect from light

Formula: C₂₁H₁₂O₇
MW: 376.32 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00036873
CAS Number: 3301-79-9
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