You Searched For: Cell Culture Supplements and Additives

Catalog Number: (536252S)

Supplier: VWR Chemicals

Description: Egg yolk tellurite emulsion, VWR®

UOM: 1 * 50 mL

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Supplier: VWR Chemicals

Description: Contains a mixture of protease inhibitors: AEBSF, Aprotinin, E-64, Bestatin and Leupeptin. Each vial can be reconstituted in 1 ml deionised water to form a 100X solution.

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Supplier: VWR Chemicals

Description: Yeast extract is the water soluble portion of autolysed yeast containing a source of Vitamin B complex.

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Catalog Number: (M250-1ML)

Supplier: VWR Chemicals

Description: Contains protease inhibitors for mammalian cell use: AEBSF, Aprotinin, E-64, Bestatin, Leupeptin and Pepstatin

UOM: 1 * 1 mL

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Supplier: VWR Chemicals

Description: VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

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Supplier: Biowest

Description: L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

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Supplier: MP Biomedicals

Description: M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of<i> E.coli</i> and plasmid amplification.

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Supplier: HyClone products (Cytiva)

Description: HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.

Supplier: G-Biosciences

Description: The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Supplier: G-Biosciences

Description: Protease-PhosphataseArrest™ provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

Supplier: PROTIDE PHARMACEUTICALS

Description: Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Catalog Number: (PRTV2170401)

Supplier: PROVITRO

Description: The supplement kit for defined fibroblast maintenance medium is suitable for maintenance culturing of Provitro human fibroblasts (HFIB) after adding the supplement kit components to 500 ml basal medium. The formulation is optimized for initial seeding of 6000 cells/cm². Feeder-layer, matrix substrates or other substances are not necessary. We recommend using the antibiotic supplement for freshly isolated cells only.

UOM: 1 * 500 mL

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Catalog Number: (L3010-005)

Supplier: Biowest

Description: Phytohaemagglutinin is a lectin extracted from red kidney beans (<i>Phaseolus vulgaris</i>). PHA-M is the mucoprotein form and is a crude extract used for the stimulation of cell proliferation in lymphocyte culture. PHA-M also has a powerful erythroagglutinating property and it was originally used for separating leukocytes from whole blood.

UOM: 1 * 5 mL

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Supplier: HyClone products (Cytiva)

Description: With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Catalog Number: (733-1948)

Supplier: G-Biosciences

Description: A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

UOM: 1 * 1 mL

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Catalog Number: (786-207)

Supplier: G-Biosciences

Description: Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.

UOM: 1 * 1 SET

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