You Searched For: Nucleic Acid Reagents

Supplier: VWR Chemicals

Description: Ready to use molecular biology grade dNTP mixes and dNTP sets. The dNTP mix is designed to save hands-on time for researchers, and reduce the possibility of contamination by reducing pipetting. dNTP solutions are also available in sets of four individual dNTPs, each 100 mm. Both are convenient for use in DNA polymerisation reactions, DNA labelling and sequencing processes.

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Catalog Number: (N224-500U)

Supplier: VWR Chemicals

Description: <i>Taq</i> DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of <i> Taq</i> DNA Polymerase to a 25 µl PCR reaction.

UOM: 1 * 500 items

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Supplier: VWR Chemicals

Description: Easy to use method for isolation up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues <1×10⁷ cells or 30 mg tissue.

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Supplier: VWR Peqlab

Description: Individually packaged deoxynucleotide triphosphates manufactured to the highest quality levels.

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Supplier: VWR Chemicals

Description: Real-Time PCR kit for detection and quantification of <i>Salmonella spp.</i> in food samples. Kit that contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of <i>Salmonella spp.</i>

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Supplier: Quantabio

Description: Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

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Supplier: Quantabio

Description: PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Supplier: OMEGA BIO-TEK

Description: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

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Supplier: Quantabio

Description: qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ <i>Taq</i> DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

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Supplier: TriLink BioTechnologies

Description: 2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve <em>in vivo</em> stability.

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Catalog Number: (ENZOENZ42817)

Supplier: ENZO LIFE SCIENCES

Description: Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for <i>in vitro</i> transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

UOM: 1 * 250 Nmo

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Supplier: TriLink BioTechnologies

Description: TriLink® 2'-O-Methyl-N6-Methyladenosine-5'-Triphosphate

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Supplier: TriLink BioTechnologies

Description: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Supplier: TriLink BioTechnologies

Description: 5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.

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Supplier: TriLink BioTechnologies

Description: TriLink® 7-Deaza-7-Propargylamino-2'-deoxyguanosine-5'-Triphosphate

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