You Searched For: Nucleic Acid Reagents

Catalog Number: (786-401)

Supplier: G-Biosciences

Description: OmniPrep™ for Mouse Tail Kit isolates high quality genomic DNA from mouse tail samples. If used according to the protocols this kit purifies DNA from 100 to 200 cm mouse tail.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Catalog Number: (700-4813)

Supplier: BTX

Description: Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.

UOM: 1 * 50 items

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Supplier: Thermo Fisher Scientific

Description: GeneJET™ Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (for example, young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, Southern blotting, and enzymatic reactions.

Catalog Number: (ICNA11EPTAG100)

Supplier: MP Biomedicals

Description: Taq-&GO™ is a ready to use optimised reaction mix for PCR.

UOM: 1 * 1 mL

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Catalog Number: (GZ-1)

Supplier: G-Biosciences

Description: Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.

UOM: 1 * 1 KIT

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Supplier: BTX

Description: The BTXpress™ is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered hard to transfect by chemical and other non viral methods. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.

Supplier: G-Biosciences

Description: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: Thermo Fisher Scientific

Description: GeneJET™ Whole Blood Genomic DNA Purification Mini Kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Supplier: Thermo Fisher Scientific

Description: GeneJET™ Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from a wide variety of plant species and tissue types.

Catalog Number: (BE-602)

Supplier: G-Biosciences

Description: G-Biosciences' GeneCAPSULE™ is an electro-elution tool for rapid, nearly 90% recovery of PCR products, DNA fragments and proteins from agarose and polyacrylamide gels

UOM: 1 * 1 KIT

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Catalog Number: (40078.)

Supplier: Biotium

Description: Digoxigenin-dUTP, alkali stable

UOM: 1 * 25 Nmo

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Supplier: SGI - DNA Synthetic Genomics

Description: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

MSDS

Supplier: MP Biomedicals

Description: Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant <i>Taq</i> DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Supplier: MP Biomedicals

Description: <i>Taq</i> DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium <i>Thermus aquaticus</i>. The recombinant form of this enzyme is expressed in <i>E. coli</i>. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

Catalog Number: (786-013)

Supplier: G-Biosciences

Description: OmniTemplate™ is specifically designed for the rapid isolation of a DNA template for PCR analysis from mammalian tissue samples, blood and cell cultures. This single tube method produces a high concentration, ready-to-use supply of DNA template for microanalysis, genotyping and a multitude of other applications.

UOM: 1 * 100 Tests

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