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You Searched For: Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Product Image-786-260

FOCUS™ SubCell, protein fractionation kit

Catalog Number: (786-260)
Supplier: G-Biosciences
Description: FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.
UOM: 1 * 1 KIT
Product Image-786-241

Ingel™ for protein digestion

Catalog Number: (786-241)
Supplier: G-Biosciences
Description: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
UOM: 1 * 1 KIT
Product Image-786-250

Protein fractionation kit, FOCUS™ signal proteins

Catalog Number: (786-250)
Supplier: G-Biosciences
Description: Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
UOM: 1 * 1 KIT
Product Image-786-077

Protein reducing agents

Supplier: G-Biosciences
Description: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Product Image-ENZOENZ450150100

EpiXtract® Nuclear protein isolation kit II (nucleic acid-free)

Catalog Number: (ENZOENZ450150100)
Supplier: ENZO LIFE SCIENCES
Description: Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.
UOM: 1 * 1 KIT

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Product Image-ENZOENZ450130002

EpiXtract® total histone extraction kit (high throughput)

Catalog Number: (ENZOENZ450130002)
Supplier: ENZO LIFE SCIENCES
Description: Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.
UOM: 1 * 1 KIT

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Product Image-PIER90065

Isobaric mass tagging kits and reagent sets, TMT™

Supplier: Thermo Fisher Scientific
Description: Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.

Product Image-30071.

Protein quantitation kit, AccuOrange™

Supplier: Biotium
Description: AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).

Product Image-786-259

Proteome kits, FOCUS™ Plant Proteome

Catalog Number: (786-259)
Supplier: G-Biosciences
Description: Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-360

Proteome kits, FOCUS™ Insect Proteome

Catalog Number: (786-360)
Supplier: G-Biosciences
Description: Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-PIER88701

Universal nuclease for cell lysis, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Image Unavailable-ENZOBMLKI1761250

Fluor-de-Lys® Developer II concentrate

Catalog Number: (ENZOBMLKI1761250)
Supplier: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL

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Product Image-786-599

Immobilised Iminobiotin Resin

Catalog Number: (786-599)
Supplier: G-Biosciences
Description: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL
Product Image-PIER90054

Endoproteinase Glu-C protease, MS grade, Pierce™

Catalog Number: (PIER90054)
Supplier: Thermo Fisher Scientific
Description: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 50 µG

MSDS


Product Image-PIER90056

Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierce™

Catalog Number: (PIER90056)
Supplier: Thermo Fisher Scientific
Description: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM: 1 * 100 µG
Product Image-PIER90053

Endoproteinase Asp-N protease, MS grade, Pierce™

Catalog Number: (PIER90053)
Supplier: Thermo Fisher Scientific
Description: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 2 µG

MSDS


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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