You Searched For: Protein Extraction

Catalog Number: (786-260)

Supplier: G-Biosciences

Description: FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.

UOM: 1 * 1 KIT

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Catalog Number: (786-241)

Supplier: G-Biosciences

Description: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

UOM: 1 * 1 KIT

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Catalog Number: (786-250)

Supplier: G-Biosciences

Description: Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Catalog Number: (ENZOENZ450150100)

Supplier: ENZO LIFE SCIENCES

Description: Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.

UOM: 1 * 1 KIT

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Catalog Number: (ENZOENZ450130002)

Supplier: ENZO LIFE SCIENCES

Description: Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Catalog Number: (ENZOBMLKI1761250)

Supplier: ENZO LIFE SCIENCES

Description: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).

UOM: 1 * 1,25 mL

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Catalog Number: (786-259)

Supplier: G-Biosciences

Description: Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.

UOM: 1 * 1 KIT

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Catalog Number: (786-360)

Supplier: G-Biosciences

Description: Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.

Supplier: Biotium

Description: AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).

Catalog Number: (786-022)

Supplier: G-Biosciences

Description: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.

UOM: 1 * 1 KIT

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Catalog Number: (786-258)

Supplier: G-Biosciences

Description: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Catalog Number: (PIER90054)

Supplier: Thermo Fisher Scientific

Description: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

UOM: 1 * 50 µG

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