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You Searched For: Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.


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Product Image-ENZOENZ42813

Bio-16-ddUTP, tetralithium salt

Catalog Number: (ENZOENZ42813)
Supplier: ENZO LIFE SCIENCES
Description: Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.
UOM: 1 * 25 Nmo

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Image Unavailable-ENZOENZ42521

Gold 550 dUTP

Catalog Number: (ENZOENZ42521)
Supplier: ENZO LIFE SCIENCES
Description: Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analogue can replace TTP in reactions in which it serves as a substrate for <i>E.coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
UOM: 1 * 25 Nmo

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Image Unavailable-ENZOENZ42843L0050

Gold 525 dUTP (lyophilised)

Catalog Number: (ENZOENZ42843L0050)
Supplier: ENZO LIFE SCIENCES
Description: Gold 525 dUTP (lyophilised)
UOM: 1 * 50 Nmo

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Image Unavailable-ENZOALX620076M001

ZnAF-2 DA

Catalog Number: (ENZOALX620076M001)
Supplier: ENZO LIFE SCIENCES
Description: Fluorescent probe for Zn²⁺. Cell permeable.
UOM: 1 * 1 mg

New Product


Image Unavailable-ENZOENZ53007C200

Organelle-ID™ RGB reagent I

Catalog Number: (ENZOENZ53007C200)
Supplier: ENZO LIFE SCIENCES
Description: Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids
UOM: 1 * 200 µl

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Image Unavailable-ENZOALX850023KI01

Phosphoserine detection set

Catalog Number: (ENZOALX850023KI01)
Supplier: ENZO LIFE SCIENCES
Description: The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.
UOM: 1 * 1 SET

New Product


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Reactive oxygen species indicators dyes

Supplier: Biotium
Description: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

MSDS

Image Unavailable-40100.

Dye conjugates dUTP CF®

Supplier: Biotium
Description: CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for <i>in situ</i> hybridisation and nucleic acid blotting applications.

Image Unavailable-92224.

Biotin SE protein labelling kit

Catalog Number: (92224.)
Supplier: Biotium
Description: This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.
UOM: 1 * 1 KIT
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Reactive quencher dyes

Supplier: Biotium
Description: Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

Image Unavailable-CAYM17170-100

Bilirubin conjugate, sodium salt

Supplier: Cayman Chemical
Description: Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.

Image Unavailable-APOSBIL3113-100ML

HRP-conjugate stabiliser, SeramunStab® STNP

Catalog Number: (APOSBIL3113-100ML)
Supplier: Apollo Scientific
Description: Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.
UOM: 1 * 100 mL
Image Unavailable-92420.

Maxi fluorescent antibody labelling kits, Mix-n-Stain™, 1 mg labelling

Supplier: Biotium
Description: The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.

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ExoBrite™ CTB EV Staining Kits, Biotium

Supplier: Biotium
Description: ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ CTB EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.

Image Unavailable-786-696

Biotin kits, Micro HOOK™

Supplier: G-Biosciences
Description: The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

Product Image-COBSD4-000

B-Phycoerythrin (BPE)

Catalog Number: (COBSD4-000)
Supplier: Columbia Biosciences
Description: Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.
UOM: 1 * 1 mg

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
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Dual use goods can only be delivered within the European Union.
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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