You Searched For: (4-Bromophenyl)trifluoroboranuide tetrabutylazanium

Catalog Number: (EDVO223/AP08)

Supplier: EDVOTEK

Description: In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish <i>Aequorea victoria</i>. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.

UOM: 1 * 1 items

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Catalog Number: (EDVO207)

Supplier: EDVOTEK

Description: This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

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Supplier: EDVOTEK

Description: Ready-to-Load™ PCR kit introduces students to the principles and applications of the Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler.

Supplier: EDVOTEK

Description: Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.

Catalog Number: (EDVO274)

Supplier: EDVOTEK

Description: Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.

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Catalog Number: (EDVO951)

Supplier: EDVOTEK

Description: Safe drinking water is vitally important to health. Both pathogenic and harmless bacteria can be found in the guts of mammals and birds. Testing water for every possible type of pathogenic bacteria is slow and costly. Thus, water is tested for a characteristic type of gut bacteria - the coliforms - including the familiar <i>E. coli</i>. Presence of coliforms is an indicator for faecal contamination. In this experiment, students test for coliforms in simulated contaminated water using colour and fluorescent reagents. They can use these same reagents to test water samples from the environment. As an extension activity, a Gram Stain test can be performed on the collected samples.

UOM: 1 * 1 KIT

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Catalog Number: (EDVO314)

Supplier: EDVOTEK

Description: Suppressor genes, such as p53, are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations of genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation.

UOM: 1 * 1 items

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Catalog Number: (EDVO153)

Supplier: EDVOTEK

Description: Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.

UOM: 1 * 1 KIT

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Catalog Number: (EDVO986)

Supplier: EDVOTEK

Description: Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.

UOM: 1 * 1 items

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Catalog Number: (EDVO300)

Supplier: EDVOTEK

Description: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

UOM: 1 * 1 KIT

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Catalog Number: (EDVO953)

Supplier: EDVOTEK

Description: Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

UOM: 1 * 1 items

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Catalog Number: (EDVO124)

Supplier: EDVOTEK

Description: This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.

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Catalog Number: (EDVO221)

Supplier: EDVOTEK

Description: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.

UOM: 1 * 1 items

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Catalog Number: (EDVOS-44)

Supplier: EDVOTEK

Description: Kit for teaching students how to use a micropipette with ease and accuracy using multi-coloured dyes.

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Catalog Number: (EDVO371)

Supplier: EDVOTEK

Description: Students can solve a crime using PCR. Plasmid DNA is provided that, when amplified by PCR, provides products that represent individual DNA profiles.

UOM: 1 * 1 KIT

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Catalog Number: (EDVO253)

Supplier: EDVOTEK

Description: In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.

UOM: 1 * 1 KIT

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