Potassium hexacyanoferrate(II) trihydrate 98.5-102.0%, yellow crystalline powder ACS
Supplier: MP Biomedicals
Potassium ferrocyanide trihydrate is a salt of the Fe(CN)₆4- ferrocyanide ion. Ferrocyanide is a reducing iron species, being oxidised from ferrous Fe(II) to ferric Fe(III).
It is used to determine the concentration of potassium permanganate, a compound often used in titrations based on redox reactions. Used in a mixture with potassium ferricyanide and phosphate buffered solution (PBS) to provide a buffer for X-Gal, which is used to cleave Beta-galactosidase, giving a bright blue visualisation where an antibody (or other molecule), conjugated to Beta-gal, has bonded to its target.
It is membrane impermeable and can be used to assess redox activity of membrane-bound structures and mechanisms of electron transport across membranes. This approach was employed to demonstrate that Potassium ferrocyanide performs reducing activation of membrane-bound dopamine β-monooxygenase from the exterior face of adrenal vesicles, providing elucidation of native reduction-activation mechanisms for dopamine β-monooxygenase regulation.
Specification Test Results
Assay | 98,5 - 102,0% |
Insoluble Matter | ≤0,005% |
Chloride | ≤0,01% |
Sulphate | Passes Test |
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