L(+)-Glutamine ≥99%, white crystalline powder
Supplier: MP Biomedicals
L-Glutamine, the uncharged and amidated analog of L-glutamic acid, is an important amino acid for the incorporation of NH4+ into biomolecules. It is biosynthesized from NH4+ and glutamate via the enzyme glutamate synthetase. In turn, degradation of glutamine to free the ammonia moiety is mediated by glutaminase. Glutamine also participates in acid-base regulation in vivo.
L-glutamine is an essential amino acid, which serves as a major energy source for cells in culture. L-glutamine is added for optimal cell performance in a culture medium ( Ghyslaine Vanier, (2007), Characterization of the invasion of porcine endothelial cells by Streptococcus suis serotype 2, Canadian Journal Of Camparative Medicine, 71(2), pages: 81-89). The utilization of glutamine under carbon starvation conditions by cultured Saccharomyces cerevisiae and its relation to the cytoplasmic compartmentation of the transcriptional activator Gln3 has been studied. The use of glutamine in cultured rat islets to probe a-ketoisocaproate oxidation has been investigated.
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Soluble in water 41300 mg/l (at 25 °C).
Upozornení: Research Use Only (RUO).
Specification Test Results
Identity Test | Passes |
Purity | ≥99% |
Residue on Ignition |
|
FTIR | Conforms to Standard |
Moisture |
|
Optical Rotation | +30 ± 5° (c = 3.5, 1N HCl) |
pH (2% aq soln) | 4.5-6.5 |
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