Isoamyl alcohol ≥99%, clear, colourless liquid
Supplier: MP Biomedicals
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Danger
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Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction.
- Japan fire code: 4.2
- One of several isomers of amyl alcohol
Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilisation in the phenol phase of long RNA molecules with long poly(A) portions. It will also help in reducing the foaming during the extraction process.Isoamyl alcohol is also used in the extraction of ethidium bromide from DNA solutions and in the radiolabeling of RNA transcripts in nuclei that have been isolated from tissue. Isoamy alcohol has various miscellaneous uses which include HPLC analysis of various pharmaceuticals and metabolites, nalysis of oxidised and reduced pyridine nucleotides and adenylates in organic phenol extracts from mitochondria, isolation of mRNA from a thermophilic cyanobacterium, Solvent for fats, resins, alkaloids, Manufacturing isoamyl (amyl) compounds, isovaleric acid, mercury fulminate, pyroxylin, artificial silk, lacquers, smokeless powders, for dehydrating celloidin solutions, for determining fat in milk etc.
Miscible with alcohol, ether, benzene, chloroform, petroleum ether, glacial acetic acid, oils.
Formula:
(CH₃)₂CHCH₂CH₂OH MW: 88,15 g/mol Melting Pt: –179 °F (lit.) |
MDL Number:
MFCD00002934 CAS Number: 123-51-3 ADR: 3,III |
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