Klenow (3'-5' exo-) (from E. coli carrying the Klenow (3'-5' exo-) gene)

Supplier: Enzo Life Sciences
ENZOENZ44001U020EA 287.1 EUR
ENZOENZ44001U020 ALEXENZ-44001-U020
Klenow (3'-5' exo-) (from E. coli carrying the Klenow (3'-5' exo-) gene)
Enzymes
Klenow (3′-5′ exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations.

  • Used for DNA labeling
  • Not for 5´→3´ or 3´→5´exonuclease activity
  • N-terminal truncated DNA Polymerase I which retains polymerase activity

It is formulated of liquid in 20 mM TRIS-HCl, pH 7,5, containing 50% glycerol, 0,1 mM EDTA and 1 mM DTT.
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