Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
20X SSC pH 7,0 (3,0 M sodium chloride, 0,3 M sodium citrate, pH 7,0) DEPC Treated is a concentrated stock solution and should be diluted appropriately with distilled, deionised water or equivalent to its final working concentration. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionised water treated with diethyl pyrocarbonate (DEPC).
This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Upozornení: This product is for research use only and is not intended for therapeutic or diagnostic applications.
