The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.
- Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals
- High sensitivity, specificity and accuracy for live cell studies
- Compatible with major components of tissue culture media (phenol red, FBS and BSA)
- Complete set of reagents, including ROS inducers and scavengers
- Validated on microscopy, flow cytometry, and microplates
Through the combination of these two specific fluorescent probes, the kit provides a simple and specific assay for the real-time measurement of global levels of reactive oxygen species (ROS), and specifically superoxide in living cells. The Total ROS/Superoxide Detection Kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension) or 2 x 96 microplate assays.
Delivery information: Kit contains Oxidative Stress Detection Reagent (Green), 300 nmoles, Superoxide Detection Reagent (Orange), 300 nmoles, ROS Inducer (Pyocyanin), 1 µmole, ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg and Wash Buffer Salts, 1 pack.
