This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.
- Directly monitors global levels of reactive oxygen species (ROS), but not superoxide in live cells
- Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals when specific inhibitors are used
- High sensitivity, specificity and accuracy for live cell studies
- Compatible with major components of tissue culture media (phenol red, FBS and BSA)
- Complete set of reagents, including ROS inducers and scavengers
- Stringently manufactured, to control and eliminate non-specific assay artifacts
The kit includes Oxidative Stress Detection Reagent (Green) as the major component. This non-fluorescent, cell-permeable total ROS detection dye reacts directly with a wide range of reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals, yielding a green fluorescent product indicative of cellular production of different ROS/RNS types.
The kit is not designed to detect superoxide, reactive chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with standard green filter (490/525 nm), or cytometrically using any flow cytometer equipped with a blue (488 nm) laser.
The Total ROS Detection Kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in solution).
Delivery information: Kit contains Oxidative Stress Detection Reagent (Green), 300 nmoles, ROS Inducer (Pyocyanin), 1 µmole, ROS Inhibitor (N-acetyl-L-cysteine), 2×10 mg and Wash Buffer Salts, 1 pack.
