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For the quantitative determination of anti-HSP70 IgG/A/M autoantibodies in human serum.
This assay allows for reproducible, accurate and precise determination of IgG , IgA and IgM antibodies (total) to human Hsp70 in serum. The ELISA kit uses recombinant human Hsp70 bound to the wells of the plate to bind anti-human Hsp60 antibodies present in human serum. The captured anti-human Hsp70 antibodies are detected with a HRP conjugated goat polyclonal antibody specific for human IgG, IgA and IgM molecules. The assay is developed with tetramethylbenzidine substrate pro ducing a blue color in proportion to the amount of captured anti -human Hsp70 antibodies. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450 nm.
Informace pro objednávání: Contains Microtiter plate, Standard, Sample diluent 2, Wash buffer concentrate, HRP Conjugate, TMB Substrate, Stop solution 2.