Proteasome 20S Assay Kit

Supplier: Enzo Life Sciences
ALEXBML-AK740-0001EA 842 EUR
ALEXBML-AK740-0001 ENZOBMLAK7400001
Proteasome 20S Assay Kit
Assays Enzyme Assays
A QuantiZyme™ Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome.

The Suc-LLVY-AMC fluorogenic peptide substrate supplied with this kit is a proteasome substrate commonly used for measuring chymotrypsin-like peptidase activity. The detection of proteolytic activity is based on the release of free AMC fluorophore and offers the following advantages: non-radioactive, convenient real-time kinetics of substrate cleavage, and excellent sensitivity. A proteasome inhibitor, epoxomicin, is included as a test inhibitor for screening purposes.

Delivery information: Kit includes Proteasome 20S (human) (purified), Suc-Leu-Leu-Val-Tyr-AMC, Proteasome Assay Buffer, Sodium Dodecyl Sulfate, Epoxomicin, AMC (7-Amino-4-methylcoumarin) Calibration Standard, Half Volume White Microplates.
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