Dithiothreitol (DTT, Cleland's reagent) ≥99.4% for biotechnology
Supplier: VWR Chemicals
0281-25GEA
402
EUR
0281-100G
0281-25G
0281-1KG
0281-5G
0281-2500
Dithiothreitol (DTT, Cleland's reagent) ≥99.4% for biotechnology
Dithiothreitol (DTT, Cleland's reagent)
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Specification Test Results
Abs.@283 nm (0.02 M, Water) | ≤0.05 |
DNase | none detected |
Loss on Drying | <0.5% |
Melting Point | 39 – 43 °C |
Oxidised DTT | <0.5% |
Protease | none detected |
Purity (-SH Content) | >99.4% |
RNase | none detected |
Solubility (5%, Water) | pass |
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