HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99%, high purity

Supplier: VWR Chemicals

0511-250GEA 279 EUR
0511-250G 0511-1KG 0511-50G
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99%, high purity
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid)

HEPES is a general purpose buffer available for biological research.


Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation and CO₂ fixation. Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates. Recommended buffer for glutamate binding assays, preventing binding to non receptor materials.

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Specification Test Results

DNase none detected
Heavy Metals (as Pb) ≤0.0005%
Iron ≤0.0005%
pKa @20 °C 7.45 – 7.65
Protease none detected
Purity (Dry Basis) >99%
Residue on Ignition <0.2%
RNase none detected

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