5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate N-succinimidyl ester (CDCFDA) ≥95% (by TLC) fluorescent pH indicator

Supplier: Biotium

90040.EA 268 EUR
90040.
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate N-succinimidyl ester (CDCFDA) ≥95% (by TLC) fluorescent pH indicator
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate N-succinimidyl ester (CDCFDA)

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.


  • λEx/λEm (of hydrolysis product at pH 7) = 504/529 nm
  • pKa 4.8
  • Cell permeability: Membrane permeant
  • Colors: Green
  • White solid soluble in DMSO or DMF
  • Store at -20°C and protect from light


The fluorescence of the dye is relatively insensitive to pH change and the labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation.

Formula: C₂₉H₁₇Cl₂NO₁₁
MW: 626,35 g/mol
Storage Temperature: Freezer
CAS Number: 147265-60-9

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