5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate N-succinimidyl ester (CDCFDA) ≥95% (by TLC) fluorescent pH indicator
Supplier: Biotium
90040.EA
268
EUR
90040.
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate N-succinimidyl ester (CDCFDA) ≥95% (by TLC) fluorescent pH indicator
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate N-succinimidyl ester (CDCFDA)
5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
- λEx/λEm (of hydrolysis product at pH 7) = 504/529 nm
- pKa 4.8
- Cell permeability: Membrane permeant
- Colors: Green
- White solid soluble in DMSO or DMF
- Store at -20°C and protect from light
The fluorescence of the dye is relatively insensitive to pH change and the labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation.
Formula:
C₂₉H₁₇Cl₂NO₁₁ MW: 626,35 g/mol Storage Temperature: Freezer |
CAS Number:
147265-60-9 |
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