5-Carboxyfluorescein ≥98% (by HPLC) fluorescent pH indicator

Supplier: Biotium

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51019.EA 160 EUR
51019.
5-Carboxyfluorescein ≥98% (by HPLC) fluorescent pH indicator
5-Carboxyfluorescein

5-Carboxyfluorescein has a pKa of 6.5 and can be used as a pH sensor. The excitation spectrum of the dye responds to pH similarly to that of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.


  • λEx/λEm (pH 9) = 492/514 nm
  • pKa 6.5
  • Extinction Coefficient (ε) 82,000 (pH 9)
  • Yellow orange solid soluble in pH>6 water
  • Store at 4°C and protect from light, especially in solution

Formula: C₂₁H₁₂O₇.xH₂O
MW: 376,32 g/mol (anhy)
Storage Temperature: Refrigerator
MDL Number: MFCD00036874
CAS Number: 76823-03-5

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