4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride) 10 mg/mL in water used in nuclear staining
Supplier: Biotium
DAPI (4′,6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions.
- Classic blue nuclear counterstain for fixed cells
- Can be used at higher concentrations to stain live cells
- λEx/λEm (with DNA) = 358/461 nm
- Yellow solution
- Store at 4°C and protect from light
Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H2O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate in powder form (catalog no. 40009). DAPI dilactate is more water soluble than the dihydrochloride form of the dye.
DAPI can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.
Formula:
C₁₆H₁₅N₅·2HCl MW: 350,25 g/mol Storage Temperature: Refrigerator |
MDL Number:
MFCD00012681 CAS Number: 28718-90-3 |
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