bisBenzimide H 33258 trihydrochloride (Hoechst 33258) 10 mg/mL in water

Supplier: Biotium

Synonyms: BBIHHoechst 33258HOE 33258

40044.EA 298 EUR
40044.
bisBenzimide H 33258 trihydrochloride (Hoechst 33258) 10 mg/mL in water
bisBenzimide H 33258 trihydrochloride (Hoechst 33258)

Hoechst dyes are cell membrane-permeant, minor groove binding blue fluorescent DNA stains. Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.


  • For ease of use and safer handling
  • λEx/λEm (with DNA) = 352/461 nm
  • Light yellow solution
  • Store at 4°C and protect from light


Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.

Formula: C₂₅H₂₄N₆O·3HCl
MW: 533,88 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00012679
CAS Number: 23491-45-4
EINECS: 245-690-6

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