bisBenzimide H 33258 trihydrochloride (Hoechst 33258) 10 mg/mL in water
Supplier: Biotium
Synonyms:
BBIH
, Hoechst 33258
, HOE 33258
Hoechst dyes are cell membrane-permeant, minor groove binding blue fluorescent DNA stains. Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.
- For ease of use and safer handling
- λEx/λEm (with DNA) = 352/461 nm
- Light yellow solution
- Store at 4°C and protect from light
Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.
Formula:
C₂₅H₂₄N₆O·3HCl MW: 533,88 g/mol Storage Temperature: Refrigerator |
MDL Number:
MFCD00012679 CAS Number: 23491-45-4 EINECS: 245-690-6 |
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