Oil red O 0,5% in isopropanol (IPA), Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Danger
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Synonyms:
CI 26125
M-Clarity™ quality level = MQ200
Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.
Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts. Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
Formula:
C₂₆H₂₄N₄O MW: 408,51 g/mol Flash Pt: 12,0 °C (closed cup) Storage Temperature: Room Temperature |
MDL Number:
MFCD00003898 ADR: 3,II |
Specification Test Results
Appearance dark red, sediment free |
Conforms |
Absorbance diluted 0.25ml to 50 ml with Isopropyl alcohol |
0.20 - 0.50 |
Lambda Max | 510 - 517 nm |
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