Oil red O 0,5% in isopropanol (IPA), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
Danger

Synonyms: CI 26125

M-Clarity™ quality level = MQ200

O1391-500MLEA 85.3 EUR
O1391-500ML O1391-250ML
Oil red O 0,5% in isopropanol (IPA), Sigma-Aldrich®
Oil red O

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.


Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts. Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Formula: C₂₆H₂₄N₄O
MW: 408,51 g/mol
Flash Pt: 12,0 °C (closed cup)
Storage Temperature: Room Temperature
MDL Number: MFCD00003898
ADR: 3,II
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Specification Test Results

Appearance
dark red, sediment free
Conforms
Absorbance
diluted 0.25ml to 50 ml with Isopropyl alcohol
0.20 - 0.50
Lambda Max 510 - 517 nm

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