Thiazole orange ~90% (dye content), Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Warning
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M-Clarity™ quality level = MQ200
Thiazole orange has been used for the staining and thereby quantification of reticulocytes.
Thiazole Orange can be tethered with peptide nucleic acid for the detection of target nucleic acid in homogeneous solution. It is a dye with, both predicted physical characteristics and the biological staining characteristics of a good reticulocyte dye, hence it is considered as a dye for reticulocyte analysis. It has massive fluorescence enhancement and high quantum yield upon binding to nucleic acids, particularly RNA. It is non precipitating and can permeates through live cell membranes.
Formula:
C₂₆H₂₄N₂O₃S₂ MW: 476,61 g/mol Melting Pt: 270 °C (dec., lit.) Storage Temperature: Room Temperature |
MDL Number:
MFCD00192499 CAS Number: 107091-89-4 |
Specification Test Results
Appearance (Colour) | Orange to Very Dark Orange and Orange-Red and Red-Orange and Dark Red-Orange |
Appearance (Form) | Powder |
Infrared spectrum | Conforms to Structure |
Wavelength | 501 - 513 nm |
Extinction Coefficient | ≥65000 |
Wavelength | 286 - 290 nm |
Extinction Coefficient | ≥10000 |
Wavelength | 235 - 239 nm |
Extinction Coefficient, Methanol | ≥28000 |
Carbon | 57.6 - 73.4% |
Nitrogen | 4.9 - 6.2% |
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