EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™
Supplier: Thermo Fisher Scientific
Pierce™
PIER21565EA
252
EUR
PIER21565
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)]
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
- Reactive groups: NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
- Membrane-permeable, allowing for intracellular crosslinking
- Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
- Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
Upozornení: For Research Use Only. Not for use in diagnostic procedures.
Formula:
C₁₈H₂₀N₂O₁₂ MW: 456,36 g/mol Storage Temperature: Refrigerator |
MDL Number:
MFCD00049057 CAS Number: 70539-42-3 |
Specification Test Results
Product Line | Pierce™ |
Labeling Method | Chemical Labeling |
Crosslinker Type | Homobifunctional |
Reactive Moiety | NHS Ester |
Spacer | Cleavable, Long |
Form | Powder |
Quantity | 1 g |
Type | Crosslinker |
Solubility | DMF, DMSO |
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