EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™

Supplier: Thermo Fisher Scientific

Pierce™
PIER21565EA 252 EUR
PIER21565
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)]

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.


  • Reactive groups: NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
  • Membrane-permeable, allowing for intracellular crosslinking
  • Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
  • Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS


Upozornení: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₁₈H₂₀N₂O₁₂
MW: 456,36 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00049057
CAS Number: 70539-42-3

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Specification Test Results

Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety NHS Ester
Spacer Cleavable, Long
Form Powder
Quantity 1 g
Type Crosslinker
Solubility DMF, DMSO

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