The microtiter plate provided in this kit has been pre-coated with an antibody specific to EPO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain EPO, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450 nm ±10 nm. The concentration of EPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
For Use with serum, plasma, and cell culture supernatants.
Assay range: 31,2 to 2000 pg/ml
Specificity: This assay has high sensitivity and excellent specificity for the detection of EPO. No significant cross-reactivity or interference between EPO and analogs was observed.
Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between EPO and all analogs, therefore, cross reactivity may still exist.
Storage: The TMB substrate, wash buffer (30X concentrate), and the stop solution should be stored at 4 °C upon receipt, while the other items should be stored at −20 °C.
Upozornení: For research use only. Not for use in diagnostic procedures.
