The PMA-Lite™ 2.0 LED photolysis device is a light-weight LED light box specifically designed for optimal photoactivation of samples treated with propidium monoazide (PMA), PMAxx™ or ethidium monoazide (EMA) in viability PCR applications.
- Uniform and maximal illumination to 18 tubes
- Internal fan to maintain temperature: ≤37 °C
- Timer for 5 to 45 minutes of photoactivation
- Universal outlet adapter included for customers outside north america
- Two-year warranty from date of purchase
The PMA-Lite™ 2.0 LED Photolysis Device was developed to offer controlled and consistent photoactivation of viability PCR samples treated with PMA, PMAxx™, or other photoreactive dyes. The device holds up to 18 tubes and contains multiple LED lights that are positioned to provide uniform and maximal illumination to all tubes. The PMA-Lite™ 2.0 is designed as an improved version over the previous PMA-Lite™ LED Photolysis Device by including an intuitive touch screen for programming and monitoring, in addition to offering more flexibility for photoactivation durations.
Viability PCR (v-PCR) merges the specificity and sensitivity of qPCR-based methods with a dead cell selective DNA binding dye such as PMAxx™, PMA, or EMA. The technique is extremely versatile and can be applied to numerous species of bacteria, eukaryotes, viruses, and archaea.
PMAxx™ and PMA are photoreactive dyes developed by Biotium to have superior dead cell selectivity over culture-based methods and the alternative EMA v-PCR dye. The dyes form covalent crosslinks with dsDNA upon exposure to intense visible light. The mechanism that underlies the distinction of dead microbes from live ones is two-fold. The DNA that is crosslinked to the dye is not efficiently amplified, and it precipitates during DNA isolation, resulting in a lower recovery of modified DNA. Because the dyes are cell membrane impermeant, when a sample containing both live and dead bacteria is treated with dye, only dead bacteria with compromised cell membranes are susceptible to DNA modification. In a real-time PCR reaction, dead cell DNA will show delayed amplification and higher Ct than live cell DNA. v-PCR permits quantitation of bacterial viability and can be used with complex, mixed-strain, or viable but non-culturable samples.
Dimension: 220×170×83 mm
Weight: 1,85 kg
Frequency Range: 50 to 60 Hz
Power Range: 100 to 240 V
Maximum Power: 60 W
LED Output Wavelength: 465 to 475 nm
