Aviva Systems Biology’s B2m ELISA kit (Mouse) (OKEH00344) is based on standard sandwich enzyme-linked immunosorbent assay technology. An antibody specific for B2m has been pre-coated onto a 96-well plate (12×8 well strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B2m is added, incubated and followed by washing. Avidin-Peroxidase conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalysed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B2m captured in the well.
- Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%.
Mean Intra-assay CV%: <3,9% (n = 20)
Mean Inter-assay CV%: <7,4% (n = 20)
Delivery information: The following reagents are the provided contents of the kit: B2m Microplate (96 Wells (12×8 Well strips)), 2 B2m Lyophilised standard, 100X Biotinylated B2m detector antibody (1×120 µl), 100X Avidin-HRP conjugate (1×120 µl), sample diluent 1×20 ml, detector antibody diluent (1×12 ml), conjugate diluent (1×12 ml), 25X Wash buffer (1×30 ml), stop solution (1×10 ml), TMB substrate (1×10 ml).
