Tricine, white powder
Supplier: MP Biomedicals
Tricine, a zwitterionic buffer, was first prepared by good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. The name tricine comes from tris and glycine from which it was derived.
It can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested. It has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. It is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.
Very soluble in water (25% w/v - clear, colorless solution). For molecular biology, treat the water with DEPC prior to adding the tricine. DEPC reacts with amino groups and may help to decompose the tricine.
It can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested. It has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. It is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
ΔpK/ΔT: −0,0211; Metal Binding Constants (log K) for 0,1 M solution at 20 °C: Mg2+: 1,2; Ca2+: 2,4; Mn2+: 2,7; Cu2+: 7,3
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Formula:
(HOCH₂)₃CNHCH₂CO₂H Melting Pt: 179…189 °C (dec., lit.) Density: 1.523 g/cm³ (19.85 °C) Storage Temperature: Ambient |
MDL Number:
MFCD00004277 CAS Number: 5704-04-1 EINECS: 227-193-6 Merck Index: 13,09724 |
Specification Test Results
| Synonyms | N-Tris-[hydroxymethyl]methyl glycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine |
| Form | White Powder |
| Storage | Store at room temperature (15 to 30 °C) |
| pH | ~4,0 - 6,0 (1 M solution) (Lit.), Buffering pH Range: 7,4 - 8,8 |
| pka | 8,1 at 25 °C (Lit.) |
| Sterilisation of Solutions | To sterilise solutions, MP recommends filter sterilisation using a 0,2 micron filter |
| Typical Working Concentration | For ATP assays using firefly luciferase= 25 mM tricine buffer |
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