The RNA viral extraction kit is designed for the rapid simultaneous purification of viral RNA from cell–free samples, solid samples, swabs and transport media.
- Quick isolation of high-quality, ready-to-use viral RNA
- No organic extraction or alcohol precipitation
- Complete removal of contaminants and inhibitors
- The extracted RNA can be used directly as template for standard PCR or RT-PCR
- Low sample volume required - 200 µl serum, plasma, cell-free biological fluids
- Compatible with real time detection test kits and other real time detection/end-point kits
The viral lysis buffer contains detergents that lyse and inactivate RNases ensuring the extraction of intact viral RNA. Then, in the presence of a chaotropic salt, viral nucleic acids bound selectively to glass fiber membrane in a special centrifuge tube.
The nucleic acids remain bound while a series of a rapid wash and spin steps removes contaminating cellular components. Finally, low salt elution removes the viral nucleic acids from the glass fiber membrane. The process does not require nucleic acids precipitation, organic solvent extractions, or extensive handling of the nucleic acids.
The RNA viral extraction kit can be used for the isolation of viral RNA from a broad range of RNA virus. However, performance cannot be guaranteed for every virus species and must be validated by the costumer.
