The microbial DNA extraction kit - bead beating is designed for a fast and efficient purification of microbial DNA (gram-negative and gram-positive bacteria, yeast and fungi).
- Bead Microtubes for efficient lysis included in combination liquid Proteinase K
- No organic extraction or alcohol precipitation
- Complete removal of contaminants and inhibitors for reliable downstream applications
- Typical yield: 5 to 25 µg genomic DNA
- Elution volume: 100 µl
- High quality DNA obtained that can be directly used in PCR, Southern, any enzymatic reaction, cloning, etc.
Microbial samples such as gram-positive bacteria, yeast and spores can be difficult to lyse due to their strong complex cell wall structures. The microbial DNA extraction kit - bead beating replaces enzymatic lysis by using mechanical disruption of cell wall structures with specialized beads. The Bead Microtubes can be used in combination with many compatible disruptive devices.
Beginning with a bead beating protocol, cells are lysed through a combination of mechanical force, heat and detergent. Appropriate DNA binding conditions to the microbial DNA columns are achieved by addition of large amounts of chaotropic salts (Binding Buffer) to the lysate.
Contaminants are removed by two efficient washing steps. Afterwards, the resulting DNA is recovered in a DNA-free Tris buffer to use for subsequent reactions.
Applications:
• For rapid purification of high-quality and ready-to-use genomic DNA from microrganisms (gram negative and gram-positive bacteria, yeast and fungi)
• Total DNA from microbial cultures
