Nitro blue tetrazolium chloride (NBT) ≥90% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY


M-Clarity™ quality level = MQ200

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Nitro blue tetrazolium chloride (NBT) ≥90% (by HPLC), Sigma-Aldrich®
Nitro blue tetrazolium chloride (NBT)

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.


  • Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells
  • It has been used as a substrate for western blot detection
  • It has been used for the staining of histological tissue sections
  • Substrate for dehydrogenases and other oxidases

Formula: C₄₀H₃₀Cl₂N₁₀O₆
MW: 817,65 g/mol
Melting Pt: 200 °C
MDL Number: MFCD00012159
CAS Number: 298-83-9
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Specification Test Results

Appearance (Colour) Yellow
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Water (by Karl Fischer) ≤4.5 %
Solvent Content (by GC) ≤10.0 %
Nitrogen
Corrected for Water and Solvent
16.4 - 17.9 %
Purity (HPLC) ≥90.0 %
Solubility (Colour)
c = 10 mg/ml; Water
Green-Yellow to Dark Yellow
Solubility (Turbidity) Clear
Recommended Retest Period 3 Years

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