4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) ≥99.0% for enzyme immunoassay, Sigma-Aldrich®

Supplier: Merck

Synonyms: 4-Nitrophenyl phosphate disodium salt hexahydrate, Phosphatase substrate, p-Nitrophenyl phosphate disodium salt hexahydrate, Phosphoric acid 4-nitrophenyl ester disodium salt hexahydrate, 4-Nitrophenyl phosphate disodium hexahydrate, pNPP disodium salt hexahydrate, di-Sodium 4-nitrophenyl phosphate hexahydrate

71768-25G 71768-5G
SIAL71768-25GEA 331 EUR
SIAL71768-25G SIAL71768-5G
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) ≥99.0% for enzyme immunoassay, Sigma-Aldrich®
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP)

Chromogenic substrate for the determination of acid and alkaline phosphatases.


The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Formula: C₆H₄NNa₂O₆P·6H₂O
MW: 371.144 g/mol
Melting Pt: 300 °C
Storage Temperature: Freezer
MDL Number: MFCD00066288
CAS Number: 333338-18-4
EINECS: 224-246-5

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