Microvascular Endothelial Cell Growth Medium, FCS, is a sterile liquid culture medium designed for cultivating human microvascular endothelial cells (HMVEC). Delivered as a basal medium, it is suitable for culturing Provitro HMVEC upon the addition of supplement mix components.
- Formulation is optimised for the initial seeding of 4000 cells/cm², allowing growth up to confluence (approximately 90%)
- Use of feeder layers, matrix substrates, or additional substances is unnecessary.
Due to the possibility of reduced proliferative activity, we recommend to use the antibiotic supplement for freshly isolated cells only. Microvascular endothelial cell proliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay.
Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency, and the population doubling time.
Storge: Store the supplemented Microvascular endothelial cell proliferation medium in a dark environment at temperatures ranging from 4 to 8 °C for a maximum duration of one month. Avoid heating the medium beyond 37 °C and refrain from using uncontrolled heat sources, such as microwave appliances. When utilising a portion of the medium, extract the required amount from the bottle and warm it appropriately. Never freeze the medium, as this could result in elevated salt concentrations due to the separation of pure water during freezing, leading to irreversible damage.
