Fibroblast Growth Medium is a sterile liquid culture medium designed for cultivating human fibroblasts (HFIB). Provided as a basal medium, it is ideal for culturing Provitro HFIB upon the addition of supplement mix components.
- optimised for initial seeding of 4000 cells/cm²
- Medium supports growth up to confluence (around 90%) without requiring feeder layers, matrix substrates, or additional substances
The use of feeder layers, matrix substrates, or other additives is not necessary. However, for freshly isolated cells we recommend incorporating the antibiotic supplement to address potential reductions in proliferative activity. Endothelial cell proliferation medium is thoroughly tested after each production.
All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency, and the population doubling time.
Storge: Store the supplemented hMSC proliferation medium in a dark environment at temperatures ranging from 4 to 8 °C for a maximum duration of one month. Avoid heating the medium beyond 37 °C and refrain from using uncontrolled heat sources, such as microwave appliances. When utilising a portion of the medium, extract the required amount from the bottle and warm it appropriately. Never freeze the medium, as this could result in elevated salt concentrations due to the separation of pure water during freezing, leading to irreversible damage.
