Phenol ≥99.0% saturated with buffer for biotechnology pH 4.5
Supplier: VWR Chemicals
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An ideal replacement for water-saturated phenol in many protocols. Offers the high purity and performance of water saturated phenol with greatly enhanced stability and safety.
- Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
- Liquid phenols offer a safer, easier alternative to crystalline phenol
- Optimised pH for the purification of RNA
- Long term stability > one year
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation.
Formula:
C₆H₅OH MW: 94,11 g/mol Boiling Pt: 182 °C |
MDL Number:
MFCD00002143 UN: 2821 ADR: 6.1,II |
Specification Test Results
O.D.@ 330 nm | ≤0.2 |
O.D.@ 405 nm | ≤0.1 |
O.D.@ 510 nm | ≤0.1 |
pH (Buffer) @25 °C | 4.1 – 4.5 |
Purity | ≥99 % |
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