Phenol ≥99.0% saturated with buffer for biotechnology pH 4.5

Supplier: VWR Chemicals

0981-400MLEA 257 EUR
0981-400ML 0981-100ML
Phenol ≥99.0% saturated with buffer for biotechnology pH 4.5
Phenol

An ideal replacement for water-saturated phenol in many protocols. Offers the high purity and performance of water saturated phenol with greatly enhanced stability and safety.


  • Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
  • Liquid phenols offer a safer, easier alternative to crystalline phenol
  • Optimised pH for the purification of RNA
  • Long term stability > one year


For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation.

Formula: C₆H₅OH
MW: 94,11 g/mol
Boiling Pt: 182 °C
MDL Number: MFCD00002143
UN: 2821
ADR: 6.1,II

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Specification Test Results

O.D.@ 330 nm ≤0.2
O.D.@ 405 nm ≤0.1
O.D.@ 510 nm ≤0.1
pH (Buffer) @25 °C 4.1 – 4.5
Purity ≥99 %

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