Light green SF yellowish, powder or crystals, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
M-Clarity™ quality level = MQ100
Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.
Light green SF is generally used as a cytoplasmic counterstain with nuclear stains like safranine and Feulgen stain. In histopatology, it is used for collagen fiber staining in Masson′s trichrome, Papanicolaou cytological polychrome stains, and Twort stain for microorganisms in tissue sections. It is a constituent of polychrome staining method used for differentiating secretory cells of the pituitary gland. Light green SF also stains the cellulose cell walls of plant cells.
Formula:
C₃₇H₃₄N₂Na₂O₉S₃ Melting Pt: 288 °C (dec., lit.) Storage Temperature: Room Temperature |
MDL Number:
MFCD00012121 CAS Number: 5141-20-8 EINECS: 225-906-5 Merck Index: 13,05506 |
Specification Test Results
Appearance (Colour) | Dark Red to Purple |
Appearance (Form) | Powder or Crystals |
Nitrogen | 2.1 - 3.5 % |
Sodium (Na) | ≥5% |
Solubility (Colour) | Dark Blue-Green to Very Dark Blue-Green to Very Dark Green and Dark Brown |
Solubility (Turbidity), 10 mg/ml, H₂O | Clear |
E1% | ≥600 |
Wavelength in H₂O | 631 - 634 nm |
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