Silver nitrate 2,5% for microscopy, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Danger
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Silver nitrate along with iodine are among several inorganic substances used to induce colour in microscopical preparations. Silver nitrate as a biological stain exploits the strong colors of colloidal metallic silver. Silver nitrate is an important component of the silver staining that is used for the detection of proteins separated by polyacrylamide gel electrophoresis. Silver nitrate is a component of the silver stain kit. It is required for image development in diamine as well as non-diamine silver staining.
In silver staining, silver ions are reduced to metallic silver, thereby giving metallic silver images.
Formula:
AgNO₃ MW: 169,87 g/mol |
MDL Number:
MFCD00003414 CAS Number: 7761-88-8 |
Specification Test Results
Appearance (Colour) | Green to Very Dark Green |
Appearance (form) | Powder or Crystals |
Argentometr. Titration | 80 - 120% |
Thin layer chromatography | Corresponds to standard |
Assay (atomic absorpt.) | Zn (theory) 8.74%, typically 3 - 9% |
Atomic absorption | Zn |
Solubility (Colour) | 0.01 g in 10 ml methanol |
Solubility (method) | 0.01 g in 10 ml methanol |
Loss on drying | ≤8.0 % |
Sulphated ash | 7 - 23 % |
Wavelength (1) (UV) | 621 - 636 nm |
Extinction (1%/1 cm) (1) (UV) | ≥800 |
Solvent (UV) | Water |
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