Auramine O 85% (dye content) certified by the Biological Stain Commission, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Warning
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M-Clarity™ quality level = MQ200
Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta. Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall. Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.
Formula:
C₁₇H₂₂ClN₃ MW: 303,84 g/mol Melting Pt: >250 °C (dec., lit.) Storage Temperature: Room Temperature |
MDL Number:
MFCD00012484 UN: 3077 ADR: 6.1,III |
Specification Test Results
Appearance (Form); Powder and/or Chunks | Conforms to Requirements |
Infrared spectrum | Conforms to Structure |
Carbon | 52.1 - 87.4 % |
Nitrogen | 10.7 - 18.0 % |
Wavelength, c = 0.005 - 0.006 g/L; Water | 429 - 435 nm |
Extinction Coefficient | ≥36000 |
Wavelength | 367 - 373 nm |
Extinction Coefficient | ≥15000 |
Wavelength | 249 - 255 nm |
Extinction Coefficient | ≥12000 |
Solubility (Turbidity), c = 1 mg/ml; 95% Ethanol | Clear to Very Hazy |
Biological Stain Commission | Conforms |
Dye Content | ≥80 % |
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