Acridine orange (hemizinc salt) 85% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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M-Clarity™ quality level = MQ200

158550-100GEA 468 EUR
158550-100G 158550-25G 158550-10G SIAL158550-2.5G
Acridine orange (hemizinc salt) 85% (dye content), Sigma-Aldrich®
Acridine orange (hemizinc salt)

Acridine orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs). Acridine orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine orange dye has been used to analyse mitochondria and lysosomal content by flow cytometry, characterise multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.


Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine orange staining method has been used to deliver more consistent data in the micronucleus test. It is used a diagnostic assay manufacturing, hematology, histology.

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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wavelength 489 - 495 nm
Extinction Coefficient ≥38000
Wavelength 285 - 291 nm
Extinction Coefficient ≥18000
Wavelength 265 - 271 nm
Extinction Coefficient ≥38000
Carbon ≥46.3%
Nitrogen ≥9.5%
ICP Major Analysis Zinc Component Confirmed
Solubility (Turbidity) Clear
Solubility (Colour) Orange to very dark orange and brown to very dark brown and red-orange and red-brown and orange-brown and very dark red-brown

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