5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP (p-toluidine salt), X-Phosphate) substrate ≥99%
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
- AP substrate—for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
- Chromogenic—no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
- Package options—choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
- Product is shipped at ambient temperature.
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes
Highlights of Pierce NBT/BCIP 1-Step Solution (Part No. 34042):
Best for immunoblotting applications
Ready-to-use single component
Sensitive with low background and minimial assay-to-assay variability
Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).
Upozornení: For Research Use Only. Not for use in diagnostic procedures.
Formula:
C₁₅H₁₅BrClN₂O₄P MW: 433,6214 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00040636 CAS Number: 6578-06-9 |
Specification Test Results
VISUAL | White to off-white crystalline solid, free of foreign matter. |
WATER CONTENT | (KF) ≤ 2% |
MOLAR ABSORPTIVITY | ≥ 5700 at ~292 nm in 10mM Diethanolamine, pH 10.3 at 25 °C |
PURITY | (HPLC) ≥ 99% |
INORGANIC PHOSPHATE | ≤ 0.1% |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...