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Product Image-K1232

PCR cloning kit, CloneJET

Supplier: Thermo Fisher Scientific
Description: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

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PCR cloning kits, StrataClone

Supplier: AGILENT
Description: The StrataClone PCR cloning kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

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Product Image-AGLS200552

Random mutagenesis kits geneMorph II

Supplier: AGILENT
Description: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

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Packaging extracts, Gigapack III

Supplier: AGILENT
Description: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Product Image-AGLS200519

Mutagenesis kits QuikChange

Supplier: AGILENT
Description: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

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Pfu DNA ligase

Catalog Number: (AGLS600191)
Supplier: AGILENT
Description: <i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
UOM: 1 * 1 items
Product Image-EL0016

T4 DNA ligase

Catalog Number: (EL0016)
Supplier: Thermo Fisher Scientific
Description: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
UOM: 1 * 1 KIT

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Product Image-K1261

LIC cloning and expression kits, aLICator™, Fermentas

Supplier: Thermo Fisher Scientific
Description: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Product Image-SYGEGA1100-4X50

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Supplier: SGI - DNA Synthetic Genomics
Description: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

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Gibson Assembly® Ultra kit

Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

MSDS

Product Image-27-0361-01

T4 DNA ligase

Catalog Number: (27-0361-01)
Supplier: Cytiva
Description: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
UOM: 1 * 50 Tests
Product Image-GA1100-10

DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

MSDS

Product Image-GA1100-10MM

Gibson Assembly® HiFi 1 Step, Master mixes

Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

MSDS

Product Image-GA2100-S

Gibson Assembly® Site directed mutagenesis kit

Catalog Number: (GA2100-S)
Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Method is a well-established assembly reaction that can be leveraged to join multiple, mutagenised DNA fragments with overlapping ends. Following mutagenesis, DNA fragments of various lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach lends itself to small and large constructs and encompasses both single and multiple insert assemblies.
UOM: 1 * 5 Tests

MSDS


Product Image-GA1200-50MM

Gibson Assembly® Ultra, Master mixes

Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

MSDS

Product Image-OXNTSQK-LSK114-XL

Ligation Sequencing Kits

Supplier: Oxford Nanopore Technologies
Description: Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.

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