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Description: TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.
Catalog Number: 25-6400-50
UOM: 1 * 1 KIT
Supplier: Cytiva


Description: PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules.
Catalog Number: 28-9180-04
UOM: 1 * 50 items
Supplier: Cytiva

MSDS


Description: PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.
Catalog Number: 28-9180-07
UOM: 1 * 50 items
Supplier: Cytiva

MSDS


Description: Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component
Catalog Number: 28-9893-34
UOM: 1 * 1 items
Supplier: Cytiva

Description: HiTrap TALON® crude 1 and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.
Catalog Number: 28-9537-67
UOM: 1 * 5 items
Supplier: Cytiva

MSDS


Description: Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
Catalog Number: 17-0618-02
UOM: 1 * 25 mL
Supplier: Cytiva

Description: CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
Catalog Number: 17-0430-01
UOM: 1 * 15 g
Supplier: Cytiva

Description: NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
Catalog Number: 17-0906-01
UOM: 1 * 25 mL
Supplier: Cytiva

Description: Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
Catalog Number: GEHE10600124
UOM: 1 * 10 items
Supplier: Cytiva


Description: Frac30 is a round fraction collector designed for use with the ÄKTA start purification system. The Frac30 is controlled via the ÄKTA start touch screen instrument display or using UNICORN start software on a computer. The Frac30 is attached to ÄKTA start for power and communication. It is not suited for standalone operation.
Catalog Number: 29-0230-51
UOM: 1 * 1 items
Supplier: Cytiva


Description: Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
Catalog Number: 17-0451-01
UOM: 1 * 25 mL
Supplier: Cytiva

Description: Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.
Catalog Number: RPN2232SK
UOM: 1 * 1 KIT
Supplier: Cytiva


Description: Union Valco F/F, PEEK, for HiScale™ 16 and 26
Catalog Number: 18-1126-32
UOM: 1 * 1 items
Supplier: Cytiva


Description: Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.
Catalog Number: 17-0090-10
UOM: 1 * 25 g
Supplier: Cytiva

Description: illustra™ MicroSpin™ S-300 HR columns designed for rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.
Catalog Number: 27-5130-01
UOM: 1 * 50 items
Supplier: Cytiva

Description: Fingertight connector 1/16" male
Catalog Number: 28-4064-07
UOM: 1 * 1 items
Supplier: Cytiva


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