You Searched For: Electrophoresis+Sequencers

Supplier: EDVOTEK

Description: Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

Supplier: Merck

Description: Bromelain from pineapple stem has been used to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants, as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay, to study its potential analgesic effect and to evaluate its hydrolysing effects on proenkephalin (PENK)-derived peptides containing enkephalin sequences.

Supplier: CONSORT

Description: A complete range of power supplies for electrophoresis applications, including DNA sequencing, flat bed iso-electric focussing, horizontal and vertical gels, electro-elution, Western blotting and semi-dry blotting.

Catalog Number: (PRSI91-717)

Supplier: ProSci Inc.

Description: Insulin-Degrading Enzyme (IDE) is a secreted enzyme that belongs to the peptidase M16 family. IDE is a large zinc-binding protease and cleaves multiple short polypeptides that vary considerably in sequence. IDE plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. IDE degrades amyloid formed by APP and IAPP. IDE may participate in the degradation and clearance of naturally secreted amyloid beta -protein by neurons and microglia. IDE, which migrates at 110 kDa during gel electrophoresis under denaturing conditions, has since been shown to have additional substrates, including the signaling peptides glucagon, TGF alpha and beta -endorphin.

UOM: 1 * 50 µG

Sale

Supplier: MP Biomedicals

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: VWR Collection

Description: Vertical gel electrophoresis system

Supplier: VWR Collection

Description: Vertical gel electrophoresis system, VWR®

Supplier: VWR Collection

Description: Horizontal gel electrophoresis system

Supplier: VWR Collection

Description: Horizontal gel electrophoresis system

Supplier: VWR Collection

Description: Horizontal gel electrophoresis system, VWR®

Supplier: VWR Collection

Description: Vertical gel electrophoresis system, VWR®

Supplier: VWR Collection

Description: Vertical gel electrophoresis system, VWR®

Supplier: VWR Collection

Description: Horizontal gel electrophoresis system

Supplier: VWR Collection

Description: Vertical gel electrophoresis system

Supplier: VWR Collection

Description: Horizontal gel electrophoresis system

Supplier: VWR Collection

Description: Horizontal gel electrophoresis system