You Searched For: GMBS+(N-Succinimidyl+4-maleimidobutyrate)

Catalog Number: (AATB1042)

Supplier: AAT BIOQUEST

Description: iFluor® 690 dyes have fluorescence excitation and emission maxima of ~685 nm and ~704 nm respectively.

UOM: 1 * 1 mg

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Catalog Number: (AATB1510)

Supplier: AAT BIOQUEST

Description: This zFluor™ serial dyes are developed to have the possibly highest photostability at a given wavelength compared to the other similar wavelength dyes.

UOM: 1 * 1 mg

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Supplier: AAT BIOQUEST

Description: SMCC is a non-cleavable and membrane permeable crosslinker with a spacer arm of 8.3 Å. It contains an amine-reactive succinimidyl ester (SE) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7 to 9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5 to 7.5 to form stable thioether bonds. The SE group of SMCC is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. SMCC is a reagent that is widely used for generating stable maleimide-activated carrier proteins that can spontaneously react with sulfhydryls. Alternatively these relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.

Catalog Number: (AATB2021)

Supplier: AAT BIOQUEST

Description: An amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

UOM: 1 * 25 mg

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Catalog Number: (PIER21857)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

UOM: 1 * 50 mg

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Catalog Number: (PIER22360)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

UOM: 1 * 50 mg

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Catalog Number: (AATB21246)

Supplier: AAT BIOQUEST

Description: 5-(and-6)-Carboxy SNARF-5F Succinimidyl Ester is an excellent amine-reactive building block that can be readily used for developing SNARF-5F pH probes.

UOM: 1 * 50 µG

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Catalog Number: (90030.)

Supplier: Biotium

Description: Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa 488 because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

UOM: 1 * 5 mg

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Catalog Number: (90034.)

Supplier: Biotium

Description: Full name: 5-Carboxytetramethylrhodamine succinimidyl ester (single isomer). Amine-reactive form of carboxytetramethylrhodamine (TAMRA) single isomer.

UOM: 1 * 5 mg

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Catalog Number: (90022.)

Supplier: Biotium

Description: 5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions.

UOM: 1 * 25 mg

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Catalog Number: (90032.)

Supplier: Biotium

Description: Amine-reactive form of 5-carboxyrhodamine 6G single isomer.

UOM: 1 * 5 mg

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Catalog Number: (AATB1050)

Supplier: AAT BIOQUEST

Description: iFluor® 597 is a unique color for fluorescence imaging and flow cytometry applications.

UOM: 1 * 1 mg

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Catalog Number: (AATB1371)

Supplier: AAT BIOQUEST

Description: iFluor® 780 dyes have fluorescence excitation and emission maxima of ~784 nm and ~808 nm respectively.

UOM: 1 * 1 mg

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Catalog Number: (AATB475)

Supplier: AAT BIOQUEST

Description: The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen).

UOM: 1 * 5 mg

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Catalog Number: (AATB1034)

Supplier: AAT BIOQUEST

Description: iFluor® 570 family has fluorescence that is pH-independent from pH 3 to 11.

UOM: 1 * 1 mg

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