Description: The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

Catalog Number: 786-453

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Catalog Number: 733-1946

UOM: 1 * 1 mL

Supplier: G-Biosciences

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Description: tri-Sodium citrate dihydrate ACS

Catalog Number: RC-040

UOM: 1 * 500 g

Supplier: G-Biosciences

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Description: The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.

Catalog Number: 786-126

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Catalog Number: BE-401

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.

Catalog Number: 786-356

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: OmniPrep™ for Gram Positive Bacteria Kit isolates high quality genomic DNA from gram-positive bacteria. If used according to the protocols, this kit purifies DNA from 100 ml gram-positive bacteria culture.

Catalog Number: 786-398

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: Traut's Reagent is used for the preparation of disulfide bridges or for the generation of sulphhydryl groups for conjugation. This is a small, water-soluble compound that reacts with primary amines under mild conditions (pH 7 to 10, 25 °C) for thiolation (sulphhydryl addition).

Catalog Number: BC95

UOM: 1 * 500 mg

Supplier: G-Biosciences

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Description: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.

Catalog Number: 786-022

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.

Catalog Number: 786-225

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: T4 GP32 is a single-stranded DNA binding protein with key role in DNA replication, recombination and repair. T4 GP32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase. It improves the efficiency of reverse transcriptase during RT-PCR as well as increases the yield of PCR products.

Catalog Number: GENO786-1996

UOM: 1 * 500 µG

Supplier: G-Biosciences

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Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Catalog Number: NLM-02

UOM: 1 * 150 µG

Supplier: G-Biosciences

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Description: GET™ Plasmid DNA Miniprep Kit isolates high quality plasmid DNA from 1 to 5 ml<i> E. coli </i>cultures. The kit utilises an enhanced DNA binding column to produce high yields of plasmid.

Catalog Number: 786-648

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

Catalog Number: 786-571

UOM: 1 * 1 KIT

Supplier: G-Biosciences

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Description: The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).

Catalog Number: GENO786-895

UOM: 1 * 500 Assays

Supplier: G-Biosciences

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Description: A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

Catalog Number: 786-501

UOM: 1 * 50 mL

Supplier: G-Biosciences

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