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Antigen retrieval reagent, IHC

Supplier: ENZO LIFE SCIENCES
Description: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

Product Image-786-793

Immobilised Ficin

Catalog Number: (786-793)
Supplier: G-Biosciences
Description: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
UOM: 1 * 5 mL
Product Image-DGA01

Optimizer blueBALLS™

Catalog Number: (DGA01)
Supplier: G-Biosciences
Description: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 items
Product Image-786-277

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Supplier: G-Biosciences
Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Product Image-PIER78510

T-PER™ Tissue Protein Extraction Reagent

Catalog Number: (PIER78510)
Supplier: Thermo Fisher Scientific
Description: Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
UOM: 1 * 500 mL

MSDS


Product Image-786-316

Cell surface protein isolation and labelling kit, HOOK™

Catalog Number: (786-316)
Supplier: G-Biosciences
Description: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.
UOM: 1 * 1 KIT
Product Image-PIER90051

Pierce™ EndoProteinase Lys-C Protease, MS Grade

Catalog Number: (PIER90051)
Supplier: Thermo Fisher Scientific
Description: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
UOM: 1 * 20 µG
Product Image-786-275

Antibody F(ab)₂ fragmentation kits

Supplier: G-Biosciences
Description: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Product Image-PIER78999

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Catalog Number: (PIER78999)
Supplier: Thermo Fisher Scientific
Description: This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
UOM: 1 * 500 mL
Product Image-PIER77610

Carrier proteins, maleimide-activated carrier proteins, Imject™ Pierce™

Catalog Number: (PIER77610)
Supplier: Thermo Fisher Scientific
Description: Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.
UOM: 1 * 1 SET
Product Image-LENZ05431203

Extraction thimbles

Supplier: Lenz Laborglas GmbH & CO.KG
Description: DURAN®, Borosilicate glass.

Product Image-PIER20195

Accessories for immobilized V-8 protease kit

Catalog Number: (PIER20195)
Supplier: Thermo Fisher Scientific
Description: <i>Staphylococcus aureus</i> V-8 protease, for protein digestion
UOM: 1 * 5 mg

Certificates


Product Image-533-0010

Extraction thimbles

Supplier: Whatman products (Cytiva)
Description: Grade 603 standard cellulose extraction thimbles, made of high quality cellulose, are available in a range of sizes suitable for use in thimble holders and automated extraction apparatus to ensure optimal fit.

Product Image-PIER89899

Ubiquitin enrichment kit, Pierce™

Catalog Number: (PIER89899)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
UOM: 1 * 1 KIT
Product Image-PIER88702

Universal nuclease for cell lysis, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Image Unavailable-ENZOBMLKI1761250

Fluor-de-Lys® Developer II concentrate

Catalog Number: (ENZOBMLKI1761250)
Supplier: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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