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Product Image-786-138PR

Accessories for protein kits

Supplier: G-Biosciences
Description: EZ-Grind™ grinding resin for the extraction of both proteins and DNA

Product Image-PIER88279

Mouse embryonic fibroblast isolation kit, Pierce™

Catalog Number: (PIER88279)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
UOM: 1 * 1 KIT
Product Image-786-259

Proteome kits, FOCUS™ Plant Proteome

Catalog Number: (786-259)
Supplier: G-Biosciences
Description: Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-681

InGel™ Blue for protein digestion

Catalog Number: (786-681)
Supplier: G-Biosciences
Description: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
UOM: 1 * 1 KIT
Product Image-786-257

FOCUS™ Yeast proteome kits

Catalog Number: (786-257)
Supplier: G-Biosciences
Description: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-256

Protein enrichment kit, FOCUS™ PhosphoRich™

Supplier: G-Biosciences
Description: FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUS™ PhosphoRich™ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.

Product Image-786-258

Proteome kits, FOCUS™ Bacterial Proteome

Catalog Number: (786-258)
Supplier: G-Biosciences
Description: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-022

Protein fractionation kit, FOCUS™ mitochondria

Catalog Number: (786-022)
Supplier: G-Biosciences
Description: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
UOM: 1 * 1 KIT
Product Image-786-273

Antibody fab fragmentation kit

Supplier: G-Biosciences
Description: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Product Image-PIER88287

Accessories for primary cell isolation kits, Pierce™

Supplier: Thermo Fisher Scientific
Description: Neuronal culture medium, Pierce

Image Unavailable-ENZOBMLPW1075A0001

Proteasome Purification Kit

Catalog Number: (ENZOBMLPW1075A0001)
Supplier: ENZO LIFE SCIENCES
Description: Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.
UOM: 1 * 10 Tests

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Product Image-786-250

Protein fractionation kit, FOCUS™ signal proteins

Catalog Number: (786-250)
Supplier: G-Biosciences
Description: Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
UOM: 1 * 1 KIT
Product Image-786-252

Albumin removal kit, AlbuminOUT™

Supplier: G-Biosciences
Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Image Unavailable-ENZOADI900198

HSF1 ELISA Kits

Catalog Number: (ENZOADI900198)
Supplier: ENZO LIFE SCIENCES
Description: The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 in cell lysates and tissue extracts from human and mouse origins. This is a Sensitive ELISA kit for this key transcription factor for heat shock protein (chaperone) research.
UOM: 1 * 1 KIT

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Product Image-786-275

Antibody F(ab)₂ fragmentation kits

Supplier: G-Biosciences
Description: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Product Image-786-277

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Supplier: G-Biosciences
Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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