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[pSer326]HSF1 ELISA Kits

Catalog Number: (ENZOADI900199)
Supplier: ENZO LIFE SCIENCES
Description: The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 phosphorylated at Ser326 in cell lysates and tissue extracts from human and mouse origins. This is a sensitive ELISA kit for the key transcription factor for heat shock protein (chaperone) research.
UOM: 1 * 1 KIT

New Product


Product Image-AGLS240243

AdEasy virus purification kit

Catalog Number: (AGLS240243)
Supplier: AGILENT
Description: The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution. Concentrated and purified viral particles can be obtained in 2 to 3 hours, in contrast to traditional CsCl gradient centrifugation which typically takes 12 to 48 hours.
UOM: 1 * 1 kt
Image Unavailable-ENZOALX850024KI01

Phosphothreonine detection set

Catalog Number: (ENZOALX850024KI01)
Supplier: ENZO LIFE SCIENCES
Description: This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.
UOM: 1 * 1 SET

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Product Image-786-132

GET™ Total RNA kit

Catalog Number: (786-132)
Supplier: G-Biosciences
Description: The GET™ Total RNA Kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA spin columns. After homogenisation, RNA is bound to the GET™ RNA spin columns and washed. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays.
UOM: 1 * 1 KIT
Image Unavailable-ENZOBMLUW99150001

Ubiquitin conjugating kit (HeLa lysate-based)

Catalog Number: (ENZOBMLUW99150001)
Supplier: ENZO LIFE SCIENCES
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
UOM: 1 * 20 Tests

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Image Unavailable-ENZOALX850025KI01

Phosphotyrosine detection set

Catalog Number: (ENZOALX850025KI01)
Supplier: ENZO LIFE SCIENCES
Description: This kit offers a set of seven different MAbs specific for tyrosine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries.
UOM: 1 * 1 SET

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Image Unavailable-M2350-01

Mag-Bind® Bacterial DNA 96 Kits

Supplier: OMEGA BIO-TEK
Description: <p>The Mag-Bind® Bacterial DNA kit allows rapid and reliable isolation of high quality genomic DNA (gDNA) from a wide variety of bacterial species. Up to 0,5 ml gram positive or gram negative bacterial culture can be processed each time. The key to the system are the Mag-Bind® particles that avidly, but reversibly, bind DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed.</p>

Product Image-M6246-01

DNA/RNA isolation, viral DNA/RNA 96 kit, Mag-Bind®

Supplier: OMEGA BIO-TEK
Description: Mag-Bind® Viral DNA/RNA kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from whole blood, serum, plasma, saliva and other body fluids. The Mag-Bind® magnetic beads technology enables purification of high quality nucleic acids that is free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can also be scaled up or down, allowing for the purification from various amounts of starting materials. The purified nucleic acid is ready for direct use in downstream applications, such as amplification or other enzymatic reactions.

MSDS

Product Image-R6830-02

RNA-Solv® reagent

Catalog Number: (R6830-02)
Supplier: OMEGA BIO-TEK
Description: RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
UOM: 1 * 200 mL

MSDS Certificates


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Phosphoserine detection set

Catalog Number: (ENZOALX850023KI01)
Supplier: ENZO LIFE SCIENCES
Description: The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.
UOM: 1 * 1 SET

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Product Image-BE-401

Experimentation kits, physical properties of proteins

Catalog Number: (BE-401)
Supplier: G-Biosciences
Description: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.
UOM: 1 * 1 KIT
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Bacterial DNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

MSDS

Product Image-786-182

Protein fractionation kit, nuclear and cytoplasmic extraction kit

Catalog Number: (786-182)
Supplier: G-Biosciences
Description: The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.
UOM: 1 * 1 KIT
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Fluor-de-Lys® Deacetylase Substrate

Catalog Number: (ENZOBMLKI1040050)
Supplier: ENZO LIFE SCIENCES
Description: Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
UOM: 1 * 50 µl

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Product Image-786-225

Total protein extraction kit, TPE™

Catalog Number: (786-225)
Supplier: G-Biosciences
Description: Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.
UOM: 1 * 1 KIT
Product Image-80-6483-37

Sample Grinding Kit

Catalog Number: (80-6483-37)
Supplier: Cytiva
Description: Sample grinding kit is ready to use for disrupting small tissue and cell samples for protein extraction.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +43 1 97002 - 0.
Dual use goods can only be delivered within the European Union.
Dual use goods can only be delivered within the European Union.
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at +43 1 97002 - 0.
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